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Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation

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Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation

Auteurs : J. Pablo Rodríguez [Chili] ; Mauricio González [Chili] ; Susana Ríos [Chili] ; Ver Nica Cambiazo [Chili]

Source :

Journal of Cellular Biochemistry [ 0730-2312 ] ; 2004-11-01.

RBID : ISTEX:EEAEC74F03B67EADDC845D9F00ABA023094960BB

Descripteurs français

Wicri :
- geographic : Chili.

English descriptors

Abstract

Human MSCs have been studied to define the mechanisms involved in normal bone remodeling and the regulation of osteogenesis. During osteogenic differentiation, MSCs change from their characteristic fibroblast‐like phenotype to near spherical shape. In this study, we analyzed the correlation between the organization of cytoskeleton of MSCs, changes in cell morphology, and the expression of specific markers (alkaline phosphatase activity and calcium deposition) of osteogenic differentiation. For osteoblastic differentiation, cells were cultured in a culture medium supplemented with 100 nM dexamethasone, 10 mM β‐ glycerophosphate, and 50 μg/ml ascorbic acid. The organization of microfilaments and microtubules was examined by inmunofluorescence using Alexa fluor 594 phalloidin and anti α‐tubulin monoclonal antibody. Cytochalasin D and nocodazole were used to alter reversibly the cytoskeleton dynamic. A remarkable change in cytoskeleton organization was observed in human MSCs during osteogenic differentiation. Actin cytoskeleton changed from a large number of thin, parallel microfilament bundles extending across the entire cytoplasm in undifferentiated MSCs to a few thick actin filament bundles located at the outermost periphery in differentiated cells. Under osteogenic culture conditions, a reversible reorganization of microfilaments induced by an initial treatment with cytochalasin D but not with nocodazole reduced the expression of differentiation markers, without affecting the final morphology of the cells. The results indicate that changes in the assembly and disassembly kinetics of microfilaments dynamic of actin network formation may be critical in supporting the osteogenic differentiation of human MSCs; also indicated that the organization of microtubules appears to have a regulatory role on the kinetic of this process. © 2004 Wiley‐Liss, Inc.

Url:

https://api.istex.fr/document/EEAEC74F03B67EADDC845D9F00ABA023094960BB/fulltext/pdf

DOI: 10.1002/jcb.20234

Affiliations:

Chili

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actin</term>
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<term>Alexa fluor</term>
<term>Alkaline</term>
<term>Alkaline phosphatase activity</term>
<term>Basal</term>
<term>Basal conditions</term>
<term>Biochem</term>
<term>Biochemical markers</term>
<term>Bone marrow</term>
<term>Calcium deposition</term>
<term>Calcium phosphate deposition</term>
<term>Cell biochem</term>
<term>Cell biochem koukouritaki</term>
<term>Cell layer</term>
<term>Cell morphology</term>
<term>Cell shape</term>
<term>Characteristic phenotype</term>
<term>Chile</term>
<term>Cortical organization</term>
<term>Culture medium</term>
<term>Cytochalasin</term>
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<term>Cytoskeletal integrity</term>
<term>Cytoskeletal organization</term>
<term>Cytoskeleton</term>
<term>Cytoskeleton organization</term>
<term>Different donors</term>
<term>Differentiation</term>
<term>Differentiation markers</term>
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<term>Human mesenchymal</term>
<term>Human mscs</term>
<term>Koukouritaki</term>
<term>Mesenchymal</term>
<term>Microtubule</term>
<term>Microtubule network</term>
<term>Morphological changes</term>
<term>Morphology</term>
<term>Msc</term>
<term>Nocodazole</term>
<term>Normal bone</term>
<term>Osteoblast</term>
<term>Osteoblastic</term>
<term>Osteogenic</term>
<term>Osteogenic conditions</term>
<term>Osteogenic differentiation</term>
<term>Osteogenic medium</term>
<term>Osteoporotic</term>
<term>Phenotype</term>
<term>Phosphatase</term>
<term>Rapid changes</term>
<term>Results show</term>
<term>Tubulin</term>
<term>Untreated</term>
<term>Untreated cells</term>
<term>Upper panels</term>
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<front><div type="abstract" xml:lang="en">Human MSCs have been studied to define the mechanisms involved in normal bone remodeling and the regulation of osteogenesis. During osteogenic differentiation, MSCs change from their characteristic fibroblast‐like phenotype to near spherical shape. In this study, we analyzed the correlation between the organization of cytoskeleton of MSCs, changes in cell morphology, and the expression of specific markers (alkaline phosphatase activity and calcium deposition) of osteogenic differentiation. For osteoblastic differentiation, cells were cultured in a culture medium supplemented with 100 nM dexamethasone, 10 mM β‐ glycerophosphate, and 50 μg/ml ascorbic acid. The organization of microfilaments and microtubules was examined by inmunofluorescence using Alexa fluor 594 phalloidin and anti α‐tubulin monoclonal antibody. Cytochalasin D and nocodazole were used to alter reversibly the cytoskeleton dynamic. A remarkable change in cytoskeleton organization was observed in human MSCs during osteogenic differentiation. Actin cytoskeleton changed from a large number of thin, parallel microfilament bundles extending across the entire cytoplasm in undifferentiated MSCs to a few thick actin filament bundles located at the outermost periphery in differentiated cells. Under osteogenic culture conditions, a reversible reorganization of microfilaments induced by an initial treatment with cytochalasin D but not with nocodazole reduced the expression of differentiation markers, without affecting the final morphology of the cells. The results indicate that changes in the assembly and disassembly kinetics of microfilaments dynamic of actin network formation may be critical in supporting the osteogenic differentiation of human MSCs; also indicated that the organization of microtubules appears to have a regulatory role on the kinetic of this process. © 2004 Wiley‐Liss, Inc.</div>
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Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation

Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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